Genome-wide expression and network analyses of mutants in key brassinosteroid signaling genes

Abstract Background Brassinosteroid (BR) signaling regulates plant growth and development in concert with other pathways. Although many genes have been identified that play a role BR signaling, the biological functional consequences of disrupting those key still require detailed investigation. Results Here we performed phenotypic transcriptomic comparisons A. thaliana lines carrying loss-of-function mutation BRI1 gene, bri1– 5, exhibits dwarf phenotype its three activation-tag suppressor were able to partially revert bri1–5 mutant WS2 phenotype, namely bri1–5/bri1–1D , bri1–5/brs1–1D, bri1–5/bak1–1D. From investigated suppressors, bri1–5/bak1–1D was most effective at transcriptional level. All suppressors showed altered expression abscisic acid (ABA signaling) pathway, indicating ABA likely contributes partial recovery wild-type these suppressors. Network analysis revealed crosstalk between phytohormone pathways, suggesting interference one hormone pathway affects In addition, differential suggested existence strong negative feedback from on biosynthesis also predicted BRS1 rather than being directly involved might be responsible for providing an optimal environment interaction ligand. Conclusions Our study provides insights into molecular mechanisms functions brassinosteroid genes, especially .